human normal colon fibroblast cell line ccd18co  (ATCC)

Journal: Animal Cells and Systems

Article Title: Bromelain effectively suppresses Kras-mutant colorectal cancer by stimulating ferroptosis

doi: 10.1080/19768354.2018.1512521

Figure Lengend Snippet: ACSL-4 induced ferroptosis. (a) Four different CRCs, CacO 2 , NCI-H508, HCT-116, and DLD-1, were cultured with different concentrations of erastin (left panel) or 5 μM erastin with or without bromelain (right panel) and cell death was analyzed. (b) Four different CRCs, CacO 2 , NCI-H508, HCT-116, and DLD-1, and normal colon cells, CCD18co, were treated with or without bromelain and the expression level of ACSL-4 was analyzed. (c) DLD-1 cells were treated with erastin, siACSL-4, or bromelain. Cell death was quantified by PI staining coupled with flow cytometry (upper panel) and lipid ROS was measured (lower panel). (d) Schematic diagram describing the action of bromelain in ferroptosis. The results are shown as the mean, with error bars representing the 95% confidence interval (lower/upper limit); * p < 0.05.

Article Snippet: Human normal colon fibroblasts CCD18co (CRL-1459, ATCC, Manassas, VA, USA) were grown in Eagle's Minimum Essential Medium (Gibco) supplemented with 10% fetal bovine serum, 1% antibiotic/anti-mycotic, 2 mM GlutaMAX, and 0.1 mM non-essential amino acids (Invitrogen).

Techniques: Cell Culture, Expressing, Staining, Flow Cytometry